GeneMarkS has used to annotate hundreds of thousand prokaryotic genomes since 2001 when the initial version of GeneMarkS became available.
John Besemer, Alexandre Lomsadze and Mark Borodovsky
GeneMarkS: a self-training method for prediction of gene starts in microbial genomes. Implications for finding sequence motifs in regulatory regions.
Nucleic Acids Research (2001) 29, pp 2607-2618
This webpage provides accesses to version 4.28 of gene prediction program GeneMarkS. This version combines the original 2001 prokaryotic GeneMarkS with later development, which extended the unsupervised gene prediction to intron-less eukaryotes, eukaryotic viruses, phages and EST/cDNA sequences.